Electric field charge polarity triggers the organization and promotes the stability of electrosprayed probiotic cells

The encapsulation and organization of Bifidobacterium animalis subsp. lactis (BIFIDO) probiotic cells within maltodextrin microcapsules using electrospray processing was investigated. By choosing an appropriate polarity the DC electric field, surface charged were localized either in core or towards capsule, as visualized confocal microscopy. Negatively encapsulated a negative on electrospraying nozzle, ‘organized’ mostly microcapsules. affected evaporation solvent (water) subsequently glass transition temperature (Tg) electrosprayed Moreover, viability significantly improved for up to 2 weeks storage at 25 °C 35% RH, when located microcapsules, compared case where probiotics distributed surface. Overall, this study presents novel process that promotes stability cells.